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Optoelectronic sensor of polarization state of light from biological samples
Mikláš, Jan ; Brüstlová, Jitka (referee) ; Tománek, Pavel (advisor)
The living body tissues consist of cells which dimensions are bigger than a wavelength of visible light. Therefore a Mie scattering of reflected of backscaterred light occurs and different polarization states arise. The changes of polarization state due to the multiple scattering of light in the biological cellular tissues allow measure the aging of biological tissue. The reflected or backscattered polarized laser light exhibits multiple scattering on the sample surface and in its subsurface area.
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Analysis of proteins and their changes in biological tissues
Rudl Kulhavá, Lucie
(EN) Proteins are the important biologically active substances for the organism, their qualitative and quantitative composition determines thein function in the organism. Protein analysis in biological material is an important part of biological and medical research. This dissertation is focused on the optimalization of sample preparation - the biological materials - namely the pre-analytical phase of sample processing and their preparation for their own analases and also introducing new procedures in theie praparation. The conditions of individual analysis approaches were always optimized by means of one- dimensional and two-dimensional gel electrophoresis and mass spectrometry. The obtained results were statistically evaluated and other possible approaches to futher research were outlined. The main biological material studied was human saliva with regard to oral health. Comparative study of human saliva was performed (human saliva obtained from caries-free and caries-susceptible people). The saliva composition of individual fractions was investigated with regard to oral health. The study was carried out to compare differences in the abundances of proteins in the saliva of caries-free and caries-susceptible persons, based on label-free mass spectrometry quantification, and to perform a gender...
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Analysis of proteins and their changes in biological tissues
Rudl Kulhavá, Lucie
(EN) Proteins are the important biologically active substances for the organism, their qualitative and quantitative composition determines thein function in the organism. Protein analysis in biological material is an important part of biological and medical research. This dissertation is focused on the optimalization of sample preparation - the biological materials - namely the pre-analytical phase of sample processing and their preparation for their own analases and also introducing new procedures in theie praparation. The conditions of individual analysis approaches were always optimized by means of one- dimensional and two-dimensional gel electrophoresis and mass spectrometry. The obtained results were statistically evaluated and other possible approaches to futher research were outlined. The main biological material studied was human saliva with regard to oral health. Comparative study of human saliva was performed (human saliva obtained from caries-free and caries-susceptible people). The saliva composition of individual fractions was investigated with regard to oral health. The study was carried out to compare differences in the abundances of proteins in the saliva of caries-free and caries-susceptible persons, based on label-free mass spectrometry quantification, and to perform a gender...
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Analysis of proteins and their changes in biological tissues
Kulhavá, Lucie ; Mikšík, Ivan (advisor) ; Coufal, Pavel (referee) ; Bílková, Zuzana (referee)
(EN) Proteins are the important biologically active substances for the organism, their qualitative and quantitative composition determines thein function in the organism. Protein analysis in biological material is an important part of biological and medical research. This dissertation is focused on the optimalization of sample preparation - the biological materials - namely the pre-analytical phase of sample processing and their preparation for their own analases and also introducing new procedures in theie praparation. The conditions of individual analysis approaches were always optimized by means of one- dimensional and two-dimensional gel electrophoresis and mass spectrometry. The obtained results were statistically evaluated and other possible approaches to futher research were outlined. The main biological material studied was human saliva with regard to oral health. Comparative study of human saliva was performed (human saliva obtained from caries-free and caries-susceptible people). The saliva composition of individual fractions was investigated with regard to oral health. The study was carried out to compare differences in the abundances of proteins in the saliva of caries-free and caries-susceptible persons, based on label-free mass spectrometry quantification, and to perform a gender...
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Optoelectronic sensor of polarization state of light from biological samples
Mikláš, Jan ; Brüstlová, Jitka (referee) ; Tománek, Pavel (advisor)
The living body tissues consist of cells which dimensions are bigger than a wavelength of visible light. Therefore a Mie scattering of reflected of backscaterred light occurs and different polarization states arise. The changes of polarization state due to the multiple scattering of light in the biological cellular tissues allow measure the aging of biological tissue. The reflected or backscattered polarized laser light exhibits multiple scattering on the sample surface and in its subsurface area.
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